Time-lapse fluorescence micrographs showing the dynamic behavior of Gfp-MinC. MinC is a division inhibitor which associates, and co-oscillates, with MinD. Pole-to-pole oscillation of MinD, in turn, requires the activity of MinE. On time average, the center of the cell is exposed to the lowest level of division inhibitor. Times are indicated in seconds. The last panel shows the cell viewed with DIC (differential interference contrast) optics. The bar in this panel represents 2 µm. Shown is a cell of strain PB114(lDB175)/pDR175DminCDE(Plac::minDE)/PlR::gfp-minC, cI857].
Cells were grown at 37oC in minimal medium supplemented with 50mM IPTG, and displayed a normal division pattern. For other details, see [1,2]
The MinE pattern