Time is indicated in seconds. The bottom panels show the filaments viewed with DIC optics. The bars represent 5 µm. Shown are cells of strain DR102(lDR122)/pDB346 [DminCDE, ftsZ
o (Plac::gfp-minD, minE
857]. Cells were grown at 30oC (resulting in repression of ftsZ
expression) in minimal medium supplemented with 37 mM IPTG. For additional details, see [1,2] The corresponding dynamics of MinE in such filaments can be viewed here
In the models, in a cell surpassing a critical size due to an inhibition of cell division, a transition occurs from one to two zones in which MinE sweeps back and forth. The positioning of the FtsZ pattern(s) follows the corresponding change in the distribution of MinD. The following simulation shows counter-phase oscillation in a medium-size filament with two potential divisions sites.