Although the out-of-phase oscillation was correctly described, it was clear that this mechanism was incompatible with the known components. There was no indication for an inhibitor. On the other hand, in the simulation shown above, there is no role for a more centrally localized component such as MinE. Without MinE, no oscillation takes place and MinD is stable and homogeneously attached to the membrane. How can a more centrally located component be responsible for the flashing up at the poles?
Modeling has revealed that these pattern-forming reactions depend on interaction of the activator-depleted substrate type
. The self-enhancing process consists in the assembly at the membrane. Bound molecules enhance the binding of more molecules. This goes on the expense of unbound molecules that can rapidly diffuse in the cytoplasm. Thus, the different ranges for the self-enhancing and the antagonistic component are straightforward in such an interaction. The simulation below shows the formation of a local maximum (red) on the expense of diffusible precursor molecules (grey).