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Transmembrane Signal Transduction

 
Project Leader:
Murray Coles
Department:
Protein Evolution - Lupas
Secretary: 
Karin Lehmann
Phone:
+49 7071 601-340
Fax:
+49 7071 601-349
Staff:
Alphabetical List

Introduction

Our group concentrates on protein structure determination, with a special focus on proteins involved in transmembrane signaling. Of central interest to our work is the HAMP domain, a widespread element in prokaryotic receptors that sense the extracellular environment. Such receptors typically couple sensory input in an extracellular sensor domain to regulate an intracellular effector module. As the sensor and effector are separated by the cell membrane, the regulation must occur by internal conformational changes in the segments linking them. The HAMP domain is frequently encountered in these linking segments – often as the first domain - and must play a crucial role in signal transduction. Despite ongoing research over the last decades, the distinct mechanisms of transmembrane signaling are not yet understood. Our research on an archaeal HAMP domain, Af1503, revealed conformational changes underlying the signaling process [1] and has shown how these changes are interpreted to modulate the activity of a downstream histidine kinase effector module [2, 3].

For the structure determination of HAMP domains and other proteins we work with Nuclear Magnetic Resonance (NMR) spectroscopy. NMR spectroscopy is a powerful tool for examining the structure, dynamics and interactions of biological macromolecules. As the proteins are observed in solution, it is an ideal technique for studying transmembrane receptors, where subtle conformational and dynamic changes are involved in signaling from extra- to intracellular domains [1]. For a better understanding of transmembrane signaling, we are undertaking structural analysis of full-length membrane-bound proteins.

Our group also has numerous internal (e.g. [4]) and external collaborations (e.g. [5, 6]) and has solved more than 20 protein solution structures. Besides we collaborate to develop methods for NMR measurement and spectral analysis [7]. The latter has resulted in novel methods for protein structure determination based on back-calculation of expectation NOESY spectra, which are the key source of NMR structural information.

 
 
 
[1] Hulko M., Berndt F., Gruber M., Linder JU., Truffault V., Schultz A., Martin J., Schultz JE., Lupas AN., Coles M. (2006) The HAMP Domain Structure Implies
Helix 
Rotation in Transmembrane Signaling. Cell 126: 929-940
PMID: 16959572
 
[2] Ferris HU., Dunin-Horkawicz S., Mondéjar LG., Hulko M., Hantke K., Martin J., Schultz JE., Zeth K., Lupas AN., Coles M. (2011) The mechanisms of HAMP-
mediated 
signaling in transmembrane receptors.  Structure. 19:378-85
PMID: 21397188
 
[3] Ferris HU., Dunin-Horkawicz S., Hornig N., Hulko M., Martin J., Schultz JE., Zeth K., Lupas AN., Coles M. (2012) Mechanism of regulation of receptor
histidine 
kinases. Structure. 20: 56-66 
PMID: 22244755
 
[4] Hartmann MD., Boichenko I., Coles M., Lupas AN., Hernandez Alvarez B. (2015) Structural Dynamics of the Cereblon Ligand Binding Domain. PLoS ONE 10(5):
e0128342
PMID: 26024445
 
[5] Khazina E., Truffault V., Büttner R., Schmidt S., Coles M., Weichenrieder O. Trimeric structure and flexibility of the L1ORF1 protein in human L1
retrotransposition.
(2011) Nature Struct. Mol. Biol. 18:1006-14
PMID: 21822284
 
[6] Eisenbeis S., Proffitt W., Coles M., Truffault V., Shanmugaratnam S., Meiler J., Höcker B. Potential of fragment recombination for rational design of
proteins. 
(2012) J. Am. Chem. Soc. 1344019-22
PMID: 22329686
 
[7] Diercks T., Truffault V., Coles M., Millet O. (2009) Diagonal-Free 3D/4D HN, HN-TROSY-NOESY-TROSY. J Am Chem Soc 132(7): 2138-9
PMID: 20121126