Advanced Python Programming
Date: 10, 17, 24, 31 January, 9, 14 February 2017, 9am-12:30pm
Location: Teaching lab 0.A.06, MPI for Developmental Biology
Tutor: Jens Bassler, Mateusz Korycinski, Mathew Divine (University of Tübingen)
Register by: 8 January 2017
In this course techniques and examples of applying Python for scientific analysis of biological data are presented. The course aims to strengthen the Python skills of the participants and let them design and implement a mid-size software project to aid in their scientific work. To this end, participants will be working on a self-chosen project, closely mentored by the tutors. Three lectures will introduce advanced concepts, building on the skills and knowledge obtained in the introductory course:
- Data visualization II
- Regular Expressions
The remaining three lectures will be chosen to address participants’ specific project goals.
PyMOL Scripting and Automation for Molecular Visualization and Analysis
Date: 16 January 2017, 10am-3pm
Location: Seminar room 0.A.01, MPI for Developmental Biology
Tutor: Thomas Holder (Schrödinger, Inc.)
Register by: 8 January 2017
Level: Intermediate (basic PyMOL skills required; experience with Python scripting beneficial)
Visualization is a key aspect in structural biology to study and present 3D molecular data. PyMOL is a powerful molecular visualization tool for making high quality figures and movies. This course teaches you how to automate interactive and non-interactive tasks, use third-party scripts, and create plugins that can be used by fellow researchers.
Electron Microscopical Techniques in Cell Biology, Microbiology und Virology
Date: 21 January, 2017 9am, 28 February – 6 March, 2017, 9am-5pm
Location: Electron microscopy facilities at MPI for Developmental Biology, Developmental Genetics at the ZMBP, Pathology, University Tuebingen
Tutors: Katharina Hipp, Y.-D. Stierhof (University of Tuebingen)
Register by: 15 January 2017
Content: On the first day, theoretical aspects of EM are covered in a series of lectures. This day is followed by three 2-day practicals at each of the EM facilities. The following topics are covered:
Note: The mandatory coordination meeting takes place on Saturday, January 21 at 9am in the lecture hall, Max Planck House.
General Concepts and Applications of NMR Spectroscopy
- Introduction and basic preparation methods for biological samples
- Chemical fixation, dehydration and plastic embedding, freeze fracture technique
- Cryo-methods for the biological electron microscope
- Immunolabelling for light and electron microscopy, antigens, antibodies, marker, preparation techniques
Date: 14 March 2017 10:00am-12noon
Location: Seminar room, MPI for Developmental Biology
Tutor: Vincent Truffault
Register by: 15 February 2017
The versatility of NMR spectroscopy has made it a widespread tool in chemistry and biochemistry. The course will first introduce the main concepts in their global form. Then NMR applications will be presented from small organic molecules up to bigger protein complexes, such as sample purity, structure determination, molecular interactions, dynamics and motions, determination of molecular weight etc.
Zebrafish Development and Genetics
Date: 23 March 2017 8:15am - 5:45pm
Location: Friedrich Miescher Laboratory
Instructor: Patrick Mueller
Register by: 2 March 2017
Level: All levels
Content: Do you want to know what your favorite gene is doing in a vertebrate system? The zebrafish has emerged as a powerful model organism to study all aspects of vertebrate embryonic development. In lectures and hands-on lab sessions, you will be introduced to the following concepts and approaches: Zebrafish development and embryo anatomy, mutants, mRNA and morpholino antisense oligonucleotide micro-injection, targeted mutagenesis, blastula transplantations, fate mapping, germ cell transplantations and germ line replacement to generate maternal-zygotic mutants, in situ hybridization, drug-induced phenotypes. Students are encouraged to bring their own samples (e.g. mRNA of their favorite gene) for overexpression studies.