This confocal microscope offers the following range of excitation lines: 405 nm, 488 nm, 515 nm, 532 nm 559 nm and 635 nm. The 532 nm line comes from a high repetition rate pulsed laser and it is used for ablation purposes and FRET microscopy. The unique feature of this microscope is that it has 2 laser scanner units that can work simultaneously allowing to scan large sample areas while bleaching or ablating in a smaller region. Thus this microscope is also ideal for ablation applications, FRET as well as FRAP experiments. Finally the system is suitable for DIC and has three PMT detectors in the reflection and one in transmission detection path.