Light Microscopy

Head: Christian Feldhaus
Assistants:Aurora Panzera, Sara Mendes
Phone:+49 7071 601-443

Introduction

The light microscopy facility offers a variety of methods and equipment to retrieve all the visible information available from biological specimens, including animals, plants and single cells, and to uncover structures or processes. Fluorescent labeling can be used to highlight specific features of a specimen. Highly sensitive detectors are required to record the weakest fluorescence.The structure or object in question is often not easily observable in the static 2-dimensional view of a single image. Confocal microscopy is one way of viewing the 3-dimensional structure of a specimen and can be used, for example, to determine whether two proteins are found in the same place. In developmental biology, we are particularly interested in observing biological processes over time, which requires viewing the specimen, such as a cell, while it is still alive. Specialized microscopes that can acquire images rapidly and without harming the specimen are also needed. For all of these tasks, the light microscopy facility possesses suitable microscopes and offers support with planning and designing experiments involving light microscopy. The facility also provides the hardware and software tools to analyze microscopic images and visualize the obtained data.

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