Post-transcriptional regulation of messenger RNA

Contents

Previous and current research

Future project and goals

Staff

Department 2 - Biochemistry

Previous and current research

For close to three decades, gene expression was thought to be mainly regulated at the transcriptional level. The discovery of RNA silencing pathways, alongside the realisation that post-transcriptional control provides conserved mechanisms by which cells can rapidly change gene expression patterns, have led to a renaissance in the field of post-transcriptional regulation. Post-transcriptional processes [e.g. mRNA processing, export, surveillance, silencing and turnover] are interlinked by the use of common factors and constitute a complex regulatory network that contributes to cell-type and organism specific gene expression patterns.

Our long-standing goal is the elucidation of the molecular mechanisms that regulate gene expression at the post-transcriptional level, using a combination of small-scale and functional genomic approaches.

Future project and goals

During its entire lifespan, the mRNA acts as a platform for the binding of numerous proteins, and exists in the cell as a ribonucleoprotein particle [mRNP]. It is the mRNP which is the actual substrate of post-transcriptional processes. Consequently, many RNA binding proteins play roles at multiple steps of the post-transcriptional pathway. In addition, enzymes involved in general mRNA degradation and proteins involved in mRNA surveillance, RNA silencing and translational repression, colocalise in discrete cytoplasmic foci known as mRNA processing bodies or P-bodies [see figure], suggesting that these processes are interlinked.

Our aims are:

1. to build a comprehensive list of effectors and interaction networks for post-transcriptional pathways in order to understand how these pathways are connected with each other and with different cellular processes;
2. to determine the contribution of these post-transcriptional processes to gene expression on a
   global scale and to identify endogenous targets;
3. to understand how the regulation of the endogenous targets leads to the complex phenotypes
   observed at the cellular and organism level when these pathways are perturbed.

Scientists involved

Heike Budde, Scientist

Carla Oliveira, Guest Scientist

Petit Alain-Pierre, Staff Scientist

Praveen Bawankar, Postdoctoral Fellow

Natalia Bercovich, Postdoctoral Fellow

Dipankar Bhandari, Postdoctoral Fellow

Mary Christie, Postdoctoral Fellow

Eric Huntzinger, Postdoctoral Fellow

Cátia Igreja, Postdoctoral Fellow

Stefanie Jonas, Postdoctoral Fellow

Tadashi Nishihara, Postdoctoral Fellow

Jiyong Su, Postdoctoral Fellow

Lara Wohlbold, Postdoctoral Fellow

Latifa Zekri, Postdoctoral Fellow

Francesco Asta, PhD Student

Andreas Boland, PhD Student

Jörg Braun, PhD Student

Ying Chen, PhD Student

Duygu Kuzuoglu, PhD Student

Belinda Loh, PhD Student

Birgit Schuster, Diplom Student

Technicians

Regina Büttner

Maria Fauser

Sigrun Helms

Catrin Weiler