Tübingen zebrafish stockcenter

The stockcenter keeps about 400 mutant zebrafish lines, which are publicly available for scientific research. Most of the mutants in the stockcenter were isolated in a screen for embryonic visible phenotypes in Tübingen between 1992-1994. In addition to mutants with embryonic visible phenotypes several dominant and recessive adult visible mutants are being kept (Haffter et al., 1996a; Haffter et al., 1996b).

From the 1,200 mutations that were analysed, the stockcenter keeps a subset as outbred lines of carrier fish. The mutant stocks include alleles of some of the 371 genes described (Tü ZF genes), as well as a number of wildtype and reference strains with visible markers. Most mutants that are not sufficiently characterized and not assigned to complementation groups ("unresolved") are only kept as frozen sperm samples. As a rule these alleles will not be made available.

The collection of mutants kept as live fish in the stockcenter is in some flux. Because of limited capacity, we will remove a portion of the current strains, particularly those which have not met with any interest from researches until now. Other strains carrying newly induced mutations may be added instead.

The stockcenter will normally provide outcrosses of mutant strains. But if you have problems with raising and keeping the lines at your institute, you might want to spend some time in our lab to carry out your experiments. Please contact the stockkeeper or the Tübingen zebrafish groups to make suitable arrangements. If you want to conduct only one particular experiment, we may also be able to provide fixed eggs/embryos.

Stockkeeper

Haffter, P., Granato, M., Brand, M., Mullins, M. C., Hammerschmidt, M., Kane, D. A., Odenthal, J., van Eeden, F. J. M., Jiang, Y.-J., Heisenberg, C.-P., Kelsh, R. N., Furutani-Seiki, M., Vogelsang, E., Beuchle, D., Schach, U., Fabian, C. and Nüsslein-Volhard, C. (1996a). The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio. Development 123: 1-36.

Haffter, P., Odenthal, J., Mullins, M. C., Lin, S., Farrell, M. J., Vogelsang, E., Haas, F., Brand, M., van Eeden, F. J. M., Furutani-Seiki, M., Granato, M., Hammerschmidt, M., Heisenberg, C.-P., Jiang, Y.-J., Kane, D. A., Kelsh, R. N., Hopkins, N. and Nüsslein-Volhard, C. (1996b). Mutations affecting pigmentation and shape of the adult zebrafish. Dev. Genes Evol. 206: 260-76.